Molecular identification and screening the antagonistic and cellulolytic activities of native strain Bacillus subtilis isolated from Myanmar marine sediment.

San San Yu, Khin Thae Marr, Wai Wai Mon, Khin Swe Aye

Abstract


In order to combat plant diseases and produce profitable agricultural crops, biocontrol agents derived from bacteria offer sustainable and eco-friendly options. Numerous fungal diseases have been successfully treated by some advantageous strains of Bacillus subtilis. The bacterial strain used in this study, designated S1-10, was isolated from a marine sediment sample. The antifungal qualities of the isolate were evaluated against eight out of ten plant-pathogenic fungi. The highest cellulolytic activity of the isolate was observed after four days of incubation. The association of the strain with Bacillus subtilis was confirmed by phylogenetic analysis based on the sequences of the gyrB and 16S rRNA regions. The NCBI has received strain S1-10, which has been assigned accession codes OR708648 for 16S and PV007904 for gyrB.


Keywords


Bacillus subtilis, marine sediment, antifungal effect, cellulolytic activity, phyloge-netic analysis.

Full Text:

View Full Text

References


Yao H, Liu S, Liu T, Ren D, Yang Q, Zhou Z, et al. Screening of marine sediment-derived microorganisms and their bioactive metabolites: a review. World Journal of Microbiology and Biotechnology. 2023;39(7):172.

Voser TM, Campbell MD, Carroll AR. How different are marine microbial natural products compared to their terrestrial counterparts? Natural Product Reports. 2022;39(1):7-19.

Sayem SA, Manzo E, Ciavatta L, Tramice A, Cordone A, Zanfardino A, et al. Anti-biofilm activity of an exopolysaccharide from a sponge-associated strain of Bacillus licheniformis. Microbial cell factories. 2011;10:1-12.

Elshaghabee FM, Rokana N, Gulhane RD, Sharma C, Panwar H. Bacillus as potential probiotics: status, concerns, and future perspectives. Frontiers in microbiology. 2017;8:1490. REFERENCE

Damalas CA, Eleftherohorinos IG. Pesticide exposure, safety issues, and risk assessment indicators. International journal of environmental research and public health. 2011;8(5):1402-19.

Islam MT. Potentials for biological control of plant diseases by Lysobacter spp., with special reference to strain SB-K88. Bacteria in agrobiology: plant growth responses. 2011:335-63.

Jin X, Song J, Ma J, Liu GQ. Thermostable β-xylosidase from Aspergillus fumigatus: Purification, characterization and potential application in lignocellulose bioethanol production. Renewable Energy. 2020;155:1425-31.

Alokika V, Kumar V, Singh B. Biochemical characteristics of a novel ethanol-tolerant xylanase from Bacillus subtilis subsp. subtilis JJBS250 and its applicability in saccharification of rice straw. Biomass Conversion and Biorefinery. 2021:1-13.

Deka D, Bhargavi P, Sharma A, Goyal D, Jawed M, Goyal A. Enhancement of cellulase activity from a new strain of Bacillus subtilis by medium optimization and analysis with various cellulosic substrates. Enzyme research. 2011;2011(1):151656.

Errington J, Aart LTvd. Microbe Profile: Bacillus subtilis: model organism for cellular development, and industrial workhorse. Microbiology. 2020;166(5):425-7.

Duan M, Zhang Y, Zhou B, Qin Z, Wu J, Wang Q, et al. Effects of Bacillus subtilis on carbon components and microbial functional metabolism during cow manure–straw composting. Bioresource Technology. 2020;303:122868.

Ntozonke N, Okaiyeto K, Okoli AS, Olaniran AO, Nwodo UU, Okoh AI. A marine bacterium, Bacillus sp. isolated from the sediment samples of Algoa Bay in South Africa Produces a Polysaccharide-Bioflocculant. International journal of environmental research and public health. 2017;14(10):1149.

Aquino-Martínez JG, Vázquez-García LM, Reyes-Reyes BG. Biocontrol in vitro e in vivo de Fusarium oxysporum Schlecht. f. sp. dianthi (Prill. y Delacr.) Snyder y Hans. Con hongos antagonistas nativos de la zona florícola de Villa Guerrero, Estado de México. Revista mexicana de fitopatología. 2008;26(2):127-37.

Hendricks CW, Doyle JD, Hugley B. A new solid medium for enumerating cellulose-utilizing bacteria in soil. Applied and environmental microbiology. 1995;61(5):2016-9.

Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. Journal of bacteriology. 1991;173(2):697-703.

Munir S, Li Y, He P, He P, He P, Cui W, et al. Bacillus subtilis L1-21 possible assessment of inhibitory mechanism against phytopathogens and colonization in different plant hosts. Pak J Agric Sci. 2018;55(4):996-1002.

Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic acids research. 1997;25(24):4876-82.

Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Molecular Biology and Evolution. 2016;33(7):1870-4. Used to assess evolutionary distances via Kimuras two-parameter model and bootstrap analysis (1,000 replicates).

Nalisha I, Muskhazli M, Nor Farizan T. Production of bioactive compounds by Bacillus subtilis against Sclerotium rolfsii. Malaysian Journal of Microbiology. 2006;2(2):19-23. REFERENCE

Fan Y, Liu K, Lu R, Gao J, Song W, Zhu H, et al. Cell-free supernatant of Bacillus subtilis reduces kiwifruit rot caused by Botryosphaeria dothidea through inducing oxidative stress in the pathogen. Journal of Fungi. 2023;9(1):127.

Qu Y, Zhu M, Liu K, Bao X, Lin J. Studies on cellulosic ethanol production for sustainable supply of liquid fuel in China. Journal of Biotechnology. 2006;1(11):1235-40.

Evans E, Musa A, Abubakar Y, Mainuna B. Nigerian indigenous fermented foods: processes and prospects. In: Mycotoxin and food safety in developing countries. IntechOpen; 2013. .

Singhania RR, Sukumaran RK, Pillai A, Prema P, Szakacs G, Pandey A. Solid state fermentation of lignocellulosic substrates for cellulase production by Trichoderma reesei NRRL 11460. Indian Journal of Biotechnology. 2006;5:332-6.




DOI: https://doi.org/10.36462/H.BioSci.202505

Refbacks

  • There are currently no refbacks.


Copyright (c) 2025 Yu SS et al..

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

...........................................................................................................................................................

Other "Highlights in" Journals

Highlights in Bioinformatics, Highlights in Chemistry, Highlights in Science, Highlights in Microbiology, Highlights in Plant Science

Free counters!


........................................................................................................................................

International Library of Science "HighlightsIn" is an Open Access Scientific Publishers, aiming to science and knowledge support