Annual variation of the biochemical composition, energy reserves and physiological indices in Mactra stultorum tissues from the Tunisian coasts

Imene Chetoui, Safa Bejaoui, Feriel Ghribi, M'hamed El Cafsi


The present study assessed the consequences of environmental changes on the biochemical quality and the physiological condition of the whole body and others organs Mactra stultorum collected from the north coast of Tunisia. Significant variations in the biochemical components, PE and CI have shown between seasons. The depletion of glycogen in all tissues was recorded during spawning periods (late spring and summer). Lipid accumulation in gonad–visceral mass during the early and late gametogenesis stages was associated with the increase in CI and GSI. However, decreases in lipid and protein contents were recorded in all the tested organs during summer when the animals are in the spawning period, suggesting that clams could accumulate proteins and lipids. Regarding the environmental factors, a negative and a significant correlation was recorded between glycogen, lipid contents and water temperature. Based on these results and as M. stultorum is appreciated by the consumers, we suggest that the harvesting of M. stultorum should be concentrated on February to June when the whole body was characterized by an important amount of the biochemical composition when the seafood is at its highest nutritive value as compared to August to January. Because of its high nutritive value especially it’s richness by some important and essentials fatty acids, M. stultorum seems to be considered as   an important commercial species in many countries and in Tunisia particularly. We suggest that harvesting of M. stultorum should be concentrated on the period when the seafood is at its highest nutritive value. survivability rates from malignancy. The greatest variations in the mortality ratios existed within the European countries.


Mactra stultorum, Seasonal variations, Biochemical composition, Percentage edibility, Condition index and Gonado-somatic index

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Massapina C, Joaquin S, Matias D, Devauchelle N. Oocyte and embryo quality in Crassostrea gigas (Portuguese strain) during a spawning period in Algarve, South Portugal. Aquat Living Resour.1999; 12:327-333.

Camacho AP, Delgado M, Fernandez-Reiriz MJ, Labarta U. Energy balance, gonad development and biochemical composition in the clam Ruditapes decussatus. Mar Ecol Prog Ser.2003; 258:133-145.

Dridi S, Romdhane M.S, El Cafsi M. Seasonal variation in weight and biochemical composition of the Pacific oyster, Crassostrea gigas in relation to the gametogenic cycle and environmental conditions of the Bizerte lagoon, Tunisia. Aquaculture. 2007; 263: 238-248.

Mathieu M ,Lubet, P. Storage tissue metabolism and reproduction in marine bivalves: a brief review. Invertebr Repro Dev.1993; 23:123-129.

Beukema JJ. Caloric values of marine invertebrates with an emphasis on the soft parts of marine bivalves. Ocean Mar Biol. 1997; 35:387-414.

Barber BJ, Blake NJ. Substrate catabolism related to reproduction in the bay scallop Argopecten irradians concentricus, as determined by O/N and RQ physiological indexes. Mar Biol. 1985; 87:13-18.

Matias A C, Marinho HS, Cyrne L, Herrero E, Antunes F. Biphasic modulation of fatty acid synthase by hydrogen peroxide in Saccharomyces cerevisiae. Arch Biochem Biophys.2011; 515: 107–111.

Gabbott PA. Developmental and seasonal metabolic activities in marine molluscs. In: Hochachka PW, editors. The Mollusca. Environ Biochem Physiol (3). New York: Academic Press; 1983.p.165–217.

Beninger PG, Lucas A. Seasonal variations in condition index, reproductive activity, and gross biochemical composition of two species of adult clam reared in a common habitat: Tapes decussatus L. (Jereys) and Tapes philippinarum (Adams and Reeve). J Exp Mar Biol Ecol. 1984; 79: 19-37.

Berthelin C, Kellner K, Mathieu M. Storage metabolism in the Pacific oyster (Crassostrea gigas) in relation to summer mortalities and reproductive cycle (west coast of France). Comp Biochem Physiol.2000; 125 (B):359-369.

Ojea J, Pazos AJ, Martinez D, Novoa S, Sanchez JL, Abad M. Seasonal variation in weight and biochemical composition of the tissues of Ruditapes decussatus in relation to the gametogenic cycle. Aquaculture.2004; 238: 451-469.

Orban E, Lena G Di, Nevigato T, Casini I, Caproni R, Santaroni G, Giulini G. Nutritional and commercial quality of the striped venus clam, Chamelea gallina, from the Adriatic sea. Food Chemistry. 2007;101:1063-1070.

Celik MY, Karayucel S, Karayucel I, Ozturk R, Eyuboglu B. Meat yield, condition index and biochemical composition of mussels (Mytilus galloprovincialis Lamarck, 1819) in Sinop, South of the Black Sea. J Aquat Food Prod Technol.2012;21:198-205.

Li Q, Yang L, Ke Q, Kong L. Gametogenic cycle and biochemical composition of the clam Mactra chinensis (Mollusca: Bivalvia): Implications for aquaculture and wild stock management. Mar Biol Res. 2011;7 : 407-415.

FAO Fiches. FAO d’identification des espèces pour les besoins de la pêche en Méditerranée et Mer Noir. Zone de pêche.1987; 33(I).p. 760.

Enzenros, Enzenros R. Untersuchungn uber das Vorkommen mariner Mollusken in Tunesichen Gewassern. In: Zmouri-Langar N, Chouba L, Ajjahi Chebil L, Mrabet R, El Abed A. editors. Les coquillages Bivalves des côtes Tunisiennes. Bull Inst Natn Scien Tech Mer de Salmmbô. Tunisienne d’Imprimerie Papetrie (TIP):Tunis ; 2001. p.128.

Chetoui I, Rabeh I, Bejaoui S, Telahigue K, Ghribi F, El Cafsi M. Seasonal first investigation in the fatty acid composition in three organs of the Tunisian bivalve Mactra stultorum. Grasas y Aceites.2019; 70(1):1-10.

Direction Départementale des affaires maritimes de la manche (DDAM), Tailles de Capture, période de pêche, engins de autorisés et quantités maximales de pêche par jour et par pécheur pour les espèces de coquillage, poissons, crustacés et céphalopodes.Manche; 2013.p. 2.

Chetoui I., Telahigue K, Bejaoui S, Rabeh I, Ghribi F, Deni, F, El Cafsi M. Annual reproductive cycle and condition index of (Mactra corallina (Mollusca: Bivalvia) from the north coast of Tunisia. Inver Reprod Dev. doi: org/10.1080/07924259.2018.1529636.

Aminot A, Chaussepied C. Manuel des analyses chimiques en milieu marin. In: Centre National d’Exploitation des Océans. CNEXO. Éditions Jouve. Brest: France; 1983. p. 395.

Lowry O H, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. ). J Exp Mar Biol Ecol.1951; 12:103-118.

Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal Chem, 1956; 28:350-356.

Folch J, Lees M, Sloane-Stanley GA. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem.1957; 226: 49-509.

Deslous-Paoli JM, Heral M, Berthome P, Razet D, Garnier J. Natural reproduction of Crassotrea gigas (Thumberg) in Marennes-Orléon basin in 1979 and 1981: Biochemical and energetic aspects. Revue des travaux de l'Institut des pêches maritimes. Nantes.1981; 45: 319-327.

Anibal J, Esteves E, Rocha C. Seasonal variations in gross biochemical composition, percent edibility and condition index of the clam Ruditapes decussatus cultivated in the Ria Formosa (South Portugal). J Shellfish Res,2011; 30:17-23.

Joaquim S, Matias D, Lopes B, Arnold WS, Gaspar MB. The reproductive cycle of white clam Spisula solida (L.) (Mollusca: Bivalvia): implications for aquaculture and wild stock management. Aquaculture.2008; 281:43-48.

Park MS, Kang CK, Lee PY. Reproductive cycle and biochemical composition for the ark shell Scapharca broughtonii (Schrenck) in a southern coastal bay of Korea. J Shellfish Res. 2001; 20:177-184.

Liu WG, Li Q, Yuan YD, Zhang SH. Seasonal variations in reproductive activity and biochemical composition of the cockle Fulvia mutica (Reeve) from eastern coast of China.J Shellfish Res. 2008; 27:405-411..

Racotta IS, Ramıre JL, Avila S, Ibarra AM. Biochemical composition of gonad and muscle in the catarina scallop, Argopecten ventricosus, after reproductive conditioning under two feeding systems. Aquaculture. 1998; 163:111-122.

Ruiz C, Abad M, Sedano F, Garcia-Martin LO, Sanchez-Lopez JL. Infuence of seasonal environmental changes on the gamete production and biochemical composition of Crassostrea gigas (Thunberg) in suspended culture in El Grove, Galicia, Spain. J Exp Mar Biol Ecol.1992;, 155:249-262.

Qiaozhen K, Qi Li. Annual dynamics of glycogen, lipids and proteins during the reproductive cycle of the surf clam Mactra veneriformis from the north coast of Shandong Peninsular, China. Inver Repro Dev.2013; 57(1): 49-60.

Qi L, Lin Y, Qiaozhen K, Lingfeng, K.Gametogenic cycle and biochemical composition of the clam Mactra chinensis (Mollusca: Bivalvia): Implications for aquaculture and wild stock management. Mar Biol Res.2011;7: 407-415.

Saxby S. A review of food availability, sea water characteristics and bivalve growth performance at coastal culture sites in temperate and warm temperate regions of the world. Fisheries Research Division WA Marine Research Laboratories. Fish Res Rep.2002;132:42.

Pazos AJ, Roman G, Acosta CP, Abad M, Sanchez JL. Seasonal changes in condition and biochemical composition of the scallop Pecten maximus L. from suspended culture in the Ria de Arousa (Galicia, N.W. Spain) in relation to environmental conditions. J Exp Mar Biol Ecol. 1997;211:169-193.

Uzaki N, Kai M, Aoyama H, Suzuki T. Changes in mortality rate and glycogen content of the Manila clam Ruditapes philippinarum during the development of oxygen-deficient waters. Fish Sci. 2003; 69: 936-943.

Unuma T, Suzuki T, Kurokawa T, Yamamoto T, Akiyama T. A protein identical to the yolk protein is stored in the testis in male red sea urchin, Pseudocentrotus depressus. Biol Bull.1998;194: 92-97.

Bayne BL, Aspects of reproduction in bivalve molluscs. In: Wiley LM, editors. Estuarine processes (1).New York: Academic Press; 1976.p. 432-448.

Lagade VM, Taware SS, and Muley DV. Seasonal variation in oxygen: Nitrogen ratio of Soletellina diphos of Bhatye estuary, Ratnagiri coast, India. J Environ Biol. 2013; 34: 123-126.

Mohite SA, Mohite AS, Singh H. On condition index and percentage edibility of the short neck clam Paphia malabarica (Chemnitz) from estuarine regions of Ratnagiri, west coast of India. Aquac Res. 2009;40: 69-73.

Rahim AA, Idris MH, Kamal AH. Wong SK, Arshad A. Analysis of condition index in Polymesoda expansa (Mousson 1849). PJBS.2012;15: 629-634.

Adjei-Boateng D, Wilson, JG. Body condition and gametogenic cycle of Galatea paradoxa (Mollusca: Bivalvia) in the Volta River estuary, Ghana. Estuar Coastal Shelfish Sci. 2011.

Ghribi F, Boussoufa D., Aouini F, Bejaoui S, Chetoui I, Rabeh I, El Cafsi M. Seasonal variation of biochemical composition of Noah's ark shells (Arca noae L. 1758) in a Tunisian coastal lagoon in relation to its reproductive cycle and environmental conditions. Aquat Living Resour.2018; 3:1-15.

Shanmugam A, Palpandi C, Sambasivam S. Some valu-able fatty acids exposed from wedge clam Donax cuneatus (Linnaeus). AFJR.2007; 1(2): 014-018.

Okumus I, Stirling HP. Seasonal variations in the meat weight, condition index and biochemical composition of mussels (Mytilus edulis L.) in suspended culture in two Scottish sea lochs. Aquaculture.1998; 159: 249-261.



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